研究室の紹介

Lab. Members

MITSUHASHI Takaaki

Research Assistant Professor (IMS)

Keywords

・Natural Product Biosynthesis
・Crystalline Sponge Method
 

ORCID

https://orcid.org/0000-0001-7728-6869
 

Biography

2014 B.S. The University of Tokyo
2019 Ph.D. The University of Tokyo
2019 Research Assistant Professor, The University of Tokyo
2020 Research Assistant Professor, Institute for Molecular Science
 

Awards

2023 Encouragement Award for Young Scientists, Division of Natural Medicines, The Pharmaceutical Society of Japan
2020 “Major Results” of Nanotechnology Platform, MEXT
2017 TERPNET2017: Best Poster Award
2017 Annual Meeting of The Japanese Society of Pharmacognosy: Best Presentation Award
2014 Annual Meeting of The Japanese Society of Pharmacognosy: Best Presentation Award
 

Original papers

  1. Jung, Y., Mitsuhashi, T.,* Kageyama, K., Kikuchi, T., Sato, S., Fujita M.* “Conformational Analysis of (+)-Germacrene D-4-ol Using the Crystalline Sponge Method to Elucidate the Origin of its Instability” Chem. Eur. J. e202400512 (2024) (*co-corresponding author)
  2. Jung, Y., Mitsuhashi, T.,* Kikuchi T., Fujita M.* “Functional Plasticity of a Viral Terpene Synthase, OILTS, that Shows Non–Specific Metal Cofactor Binding and Metal–dependent Biosynthesis” Chem. Eur. J. e202304317 (2024) (*co-corresponding author)
  3. Jung, Y., Mitsuhashi, T.,* Sato, S., Senda, M., Senda, T., Fujita M.* “Function and Structure of a Terpene Synthase Encoded in a Giant Virus Genome” J. Am. Chem. Soc., 145, 25966–25970 (2023) (*co-corresponding author) Highlighted on Nature Catalysis and Science IN THE PIPELINE
  4. Zhou, B., Utjapimuk, S., Yan, K., Dubey, R., Kikuchi, T., Mitsuhashi, T., Fujita, M. “Rapid Analysis of Trace Amounts of Amino Acid Derivatives by a Formyl Group-Installed Crystalline Sponge” Chem. Asian. J., Early View, e202300969 (2023)
  5. Chen, J., Kikuchi, T., Takagi, K., Kiyota, H., Adachi, K., Mitsuhashi, T., Fujita, M. “Structure analysis of polyhalogenated persistent organic pollutants by the crystalline sponge method.” Chem. Lett., 51, 85–87 (2022)
  6. Mori, T., Nakashima, Y., Chen, H., Hoshino, S., Mitsuhashi, T., Abe, I. “Structure-based redesign of Fe(ii)/2-oxoglutarate-dependent oxygenase AndA to catalyze spiro-ring formation.” Chem. Commun., 58, 5510–5513 (2022)
  7. Wada, N., Kageyama, K., Jung, Y., Mitsuhashi, T., Fujita, M. “Solvent effects in the crystalline sponge method: importance of co-solvents for ordering absorbed guests.” Org. Lett., 23, 9288–29291(2021)
  8. Mitsuhashi, T.,# Barra, L.,# Powers, Z.,# Kojasoy, V.,# Cheng, A., Yang, F., Taniguchi, Y., Kikuchi, T., Fujita, M., Tantillo, D. J., Porco, J. A. Jr., Abe, I. “Exploiting the potential of meroterpenoid cyclases to expand the chemical space of fungal meroterpenoids.” Angew. Chem. Int. Ed., 59, 23772–23781 (2020) (#co-first author) chosen as Hot Paper
  9. Morita, I.,# Mori, T.,# Mitsuhashi, T.,# Hoshino, S.,# Taniguchi, Y., Kikuchi, T., Nagae, K., Nasu, N., Fujita, M., Ohwada, T., Abe, I. “Exploiting a C–N bond forming cytochrome P450 monooxygenase for C–S bond formation.” Angew. Chem. Int. Ed., 59, 3988–3993 (2020) (#co-first author) chosen as Hot Paper
  10. Chen, Q., Li, J., Liu, Z., Mitsuhashi, T., Zhang, Y., Liu, H. Ma, Y., He, J., Shinada, T., Sato, T., Wang, Y., Liu, H., Abe, I., Zhang, P., Wang, G. “Molecular basis for sesterterpene diversity produced by plant terpene synthases.” Plant Commun., 1, 100051 (2020)
  11. Hoshino, S., Mitsuhashi, T., Kikuchi, T., Wong, C. P., Morita, H., Awakawa, T., Fujita, M., Abe, I. “Structural elucidation of tenebrathin: cytotoxic c-5-substituted γ-pyrone with a nitroaryl side chain from Streptoalloteichus tenebrarius.” Org. Lett., 21, 6519–6522 (2019)
  12. Powers, Z., Scharf, A., Cheng, A., Yang, F., Himmelbauer, M., Mitsuhashi, T., Barra, L., Taniguchi, Y., Kikuchi, T., Fujita, M., Abe, I., Porco, J. A. Jr. “Biomimetic synthesis of meroterpenoids by dearomatization‐driven polycyclization.” Angew. Chem. Int. Ed., 58, 16141–16146 (2019)
  13. Sato, H., Mitsuhashi, T., Yamazaki, M., Abe, I., Uchiyama, M. “Inherent atomic mobility changes in carbocation intermediates during the sesterterpene cyclization cascade.” Beilstein J. Org. Chem., 15, 1890–1897 (2019)
  14. Nakashima, Y.,# Mitsuhashi, T.,# Matsuda, Y.,# Senda, M., Sato, H., Yamazaki, M., Uchiyama, M., Senda, T., Abe, I. “Structural and computational bases for dramatic skeletal rearrangement in anditomin biosynthesis.” J. Am. Chem. Soc., 140, 9743–9750 (2018) (#co-first author)
  15. Mitsuhashi, T., Kikuchi, T., Hoshino, S., Ozeki, M., Awakawa, T., Shi, S.-P., Fujita, M., Abe, I. “Crystalline sponge method enabled the investigation of a prenyltransferase-terpene synthase chimeric enzyme, whose product exhibits broadened NMR signals.” Org. Lett., 20, 5606–5609 (2018)
  16. Sato, H., Mitsuhashi, T., Yamazaki, M., Abe, I., Uchiyama, M. "Computational studies on biosynthetic carbocation rearrangements leading to quiannulatene: initial conformation regulates biosynthetic route, stereochemistry, and skeleton type." Angew. Chem. Int. Ed., 57, 14752–14757 (2018)
  17. Mitsuhashi, T.,# Rinkel, J.,# Okada, M., Abe, I., Dickschat, J. S. “Mechanistic characterization of two chimeric sesterterpene synthases from Penicillium.” Chem. Eur. J., 23, 10053–10057 (2017) (#co-first author)
  18. Mitsuhashi, T., Okada, M., Abe, I. “Identification of chimeric αβγ diterpene synthases possessing both type II terpene cyclase and prenyltransferase activities.” ChemBioChem, 18, 2104–2109 (2017) chosen as Very Important Paper
  19. Okada, M.,# Matsuda, Y.,# Mitsuhashi, T., Hoshino, S., Mori, T., Nakagawa, K., Quan, Z., Qin, B., Zhang, H., Hayashi, F., Kawaide, H., Abe, I. “Genome-based discovery of an unprecedented cyclization mode in fungal sesterterpenoid biosynthesis.” J. Am. Chem. Soc., 138, 10011–10018 (2016) (#equall contribution)
  20. Matsuda, Y.,# Mitsuhashi,T.,# Lee, S.,# Hoshino, M., Mori, T., Okada, M., Zhang, H., Hayashi, F., Fujita, M., Abe, I. “Astellifadiene: structure determination by NMR spectroscopy and crystalline sponge method, and elucidation of its biosynthesis.” Angew. Chem. Int. Ed., 55, 5785–5788 (2016) (#co-first author)
  21. Qin, B.,# Matsuda, Y.,# Mori, T., Okada, M., Quan, Z., Mitsuhashi, T., Wakimoto, T., Abe, I. “An unusual chimeric diterpene synthase from Emericella variecolor and its functional conversion into a sesterterpene synthase by domain swapping.” Angew. Chem. Int. Ed., 55, 1658–1661 (2016) (#equall contribution)
  22. Matsuda, Y.,# Quan, Z.,# Mitsuhashi, T., Li, C., Abe, I. “Cytochrome P450 for citreohybridonol synthesis: oxidative derivatization of the andrastin scaffold.” Org. Lett., 18, 296–299 (2016) (#equall contribution)
  23. Matsuda, Y.,# Mitsuhashi, T.,# Quan, Z., Abe, I. “Molecular basis for stellatic acid biosynthesis: a genome mining approach for discovery of sesterterpene synthases.” Org. Lett., 17, 4644–4647 (2015) (#co-first author)

Reviews and Books

  1. Mitsuhashi, T., Abe, I. “Chimeric terpene synthases possessing both terpene cyclization and prenyltransfer activities.” ChemBioChem, 19, 1106–1114 (2018)
  2. Mitsuhashi, T., Abe, I. “Sesterterpenoids.” Progress in the Chemistry of Organic Natural Products, 111, 1–79 (2020)
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